Sepax HPLC Columns

• Reverse Phase
• Normal Phase
• Polar HILIC
• Polymer Phase
• UHPLC Phase

Reverse Phase

Phase Name	Silica	Modification	Particle Size		Pore Size	Pore Volume	Carbon Content	USP	Surface Area
Sepax GP-C18	Spherical, high purity (<10 ppm metals)	Monomeric and fully endcapped	1.8, 3, 4, 5, 7, and 10 µm	2.0-8.0	120 Å		17%	L1	300 m²/g
Sepax BR-C18	Spherical, high purity (<10 ppm metals)	Monomeric and fully endcapped	3, 5, and 10 µm	1.5-10.5	120 Å	1.0ml/g	19%	L1	350 m²/g
Sepax HP-C18	Spherical, high purity (<10 ppm metals)	Monomeric and fully endcapped, Compatible with 100% aqueous mobile phase	1.8, 3, 4, 5, 7, and 10 µm	2.0-8.0	120 Å	1.0ml/g	17%	L1	300 m²/g
	Spherical, high purity (<10 ppm metals)	Monomeric and fully endcapped	3 and 5 µm	2.0-8.0	200 Å	1.0ml/g	10	L1	200 m²/g
Sepax HP-C18(2)	Spherical, high purity (<10 ppm metals)	Special bonding density and proprietary endcapping	3 and 5 µm	2.0-8.0	100 Å	1.1mlg	17	L1	450 m²/g
Sepax Bio-C18	Spherical, high purity (<10 ppm metals)	Monomeric and fully endcapped	3, 5, and 10 µm	2.0-8.0	200 Å	1.0ml/g	10	L1	200 m²/g
	Spherical, high purity (<10 ppm metals)	Monomeric and fully endcapped	3, 5, and 10 µm	2.0-8.0	300 Å	0.95ml/g	7	L1	105 m²/g
Sepax GP-C8	Spherical, high purity (<10 ppm metals)	Monomeric and fully endcapped	3, 5, and 10 µm	2.0-7.0	120 Å	1.0ml/g	11%	L7	300 m²/g
Sepax Bio-C8	Spherical, high purity (<10 ppm metals)	Monomeric and fully endcapped	3, 5, and 10 µm	2.0-7.0	300 Å	0.95ml/g	4%	L7	105 m²/g
Sepax GP-C4	Spherical, high purity (<10 ppm metals)	Monomeric and fully endcapped	3, 5, and 10 µm	2.0-7.0	120 Å	1.0ml/g	8%	L26	300 m²/g
Sepax Bio-C4	Spherical, high purity (<10 ppm metals)	Monomeric and fully endcapped	3, 5, and 10 µm	2.0-7.0	300 Å	1.0ml/g	3%	L26	105 m²/g
Sepax GP-Phenyl	Spherical, high purity (<10 ppm metals)	Monomeric and fully endcapped	3, 5 µm	2.0-7.0	120 Å	1.0ml/g	11%	L11	300 m²/g

Normal Phase

Phase Name	Silica	Modification	Particle Size		Pore Size	Pore Volume	Carbon Content	USP	Surface Area
Sepax HP-Amino	Spherical, high purity (<10 ppm metals)	Monomeric and fully endcapped	3, 5 µm	2.0-7.0	120 Å	1.0ml/g	4%	L8	300 m²/g
Sepax HP-Cyano	Spherical, high purity (<10 ppm metals)	Monomeric and fully endcapped	1.8, 2.2, 3, 4, 5, and 10 µm	2.0-7.0	120 Å	1.0ml/g	7%	L10	300 m²/g
Sepax HP-Diol	Spherical, high purity (<10 ppm metals)	Monomeric and fully endcapped	3, 5, and 10 µm	2.0-7.0	80 & 120 Å	1.0ml/g	8.8%	L20	300 m²/g
Sepax HP-Silica	Sperical, high purity (<10 ppm metals)	activated hydroxyl (-OH)	1.8, 2.2, 3, 5, and 10 µm	2.0-7.0	60, 120, 200, 300, 500, 1000 and 2000 Å	1.0 ml/g for 120 Å pore size		L3	300 m²/g for 120 Å pore size

Mixed Mode Phase

Phase Name	Silica	Modification	Particle Size	pH Stability	Pore Size	Pore Volume	Carbon Content	USP	Surface Area
Sepax HP-SCX	Spherical, high purity (<10 ppm metals)	Polymeric and mixed mode	1.8, 2.2, 3, 4, 5, 7, 10 µm	1.5-8.0	120 Å	1.0ml/g	11%	L9	300 m²/g
Sepax HP-SAX	Spherical, high purity (<10 ppm metals)	Polymeric and mixed mode	3, 4, 5, 7, 10 µm	1.5-8.0	120 Å	1.0ml/g	16%	L14	300 m²/g

Polymer Based Reverse Phase HPLC

Phase Name	PS/DVB Particles	Separation Mechanism	Particle Size	pH Stability	Pore Size	Pore Volume	Carbon Content	USP	Surface Area
PolyRp	Spherical, 80% cross-linking	hydrophobic interaction	5, 10 µm	1.0-14.0	100,300,500,1000 Å	1.0ml/g	0%	L21	280 m²/g for 100A
PolyRp	Spherical, 80% cross-linking	hydrophobic interaction	1.7,3, 5, 10 µm	1.0-14.0	Non Porus	1.0ml/g	0%	L21	<10 m²/g

Polar HILIC Phase

Phase Name	Silica	Modification	Particle Size	pH Stability	Pore Size	Pore Volume	Carbon Content	USP	Surface Area
Polar Silica	Spherical, high purity (<10 ppm metals)	Monomeric and fully endcapped	1.8, 2.2, 3, 4, 5, 10 µm	1.5-8.0	120 Å	1.0ml/g	0%		300 m²/g
Polar 100	Spherical, high purity (<10 ppm metals)	Monomeric and fully endcapped	1.8, 2.2, 3, 4, 5, 10 µm	1.5-8.0	120 Å	1.0ml/g	16%		300 m²/g
Polar Diol	Spherical, high purity (<10 ppm metals)	Monomeric and fully endcapped	1.8, 2.2, 3, 4, 5, 10 µm	1.5-8.0	120 Å	1.0ml/g	8.8%		300 m²/g
Polar Pyridine	Spherical, high purity (<10 ppm metals)	Monomeric and fully endcapped	1.8, 2.2, 3, 4, 5, 10 µm	1.5-8.0	120 Å	1.0ml/g	8%		300 m²/g
Polar ImidazolE	Spherical, high purity (<10 ppm metals)	Monomeric and fully endcapped	1.8, 2.2, 3, 4, 5, 10 µm	1.5-8.0	120 Å	1.0ml/g	8.6%		300 m²/g

UHPLC Columns

Phases	Particle Size	Pore Size	Surface Area	Carbon Loading	pH Range
GP-C18	1.8, 2.2	120	300	17.0%	2-8.5
BR-C18	1.8, 2.2	120	300	19.0%	2-8.5
GP-C8	1.8, 2.2	120	300	11.0%	2-8.5
GP-C4	1.8, 2.2	120	300	8.0%	2-8.5
GP-Phenyl	1.8, 2.2	120	300	11.0%	2-8.5
HP-Amino	1.8, 2.2	120	300	7.0%	2-8.5
HP-Cyno	1.8, 2.2	120	300	4.0%	2-8.5
HP-SCX	1.8, 2.2	120	300	11.0%	2-8.5
HP-SAX	1.8, 2.2	120	300	16.0%	2-8.5
HP-Silica	1.8, 2.2	120	300	0.0%	2-8.5
Polar -100	1.8, 2.2	120	300	11.0%	2-8.5
PolyRP	1.7	Non-Porus			1-14